WebAdd 2 mL BioLegend Cell Staining Buffer (Cat No 420241). 8. Centrifuge at 350Xg for five minutes. Discard supernatant. 9. Resuspend cell pellet in 0.3-0.5 mL Cell Staining Buffer. 10. Analyze cells immediately by flow cytometry … WebMar 30, 2016 · Inhibitors and vehicle controls were added at the indicated times after which cells were washed with 500 uL of cell staining buffer from Biolegend ® (San Diego, CA, USA) (product #: 420241) and spun down at 2500 RPM for 3 min. Cells were resuspended in 100 uL of cell staining buffer prior to the addition of 5 uL of either PE mouse IgG1, κ ...
FluoroFix Buffer - punchout.biolegend.com
WebWash 3X with BioLegend's Cell Staining Buffer (Cat. No. 420241) and then resuspend the cells at the concentration of 0.5-10 x 10 6 /ml. Mix 100µl cell suspension with proper … Web1. Obtain desired tissue (e.g. spleen, lymph node, thymus, bone marrow) and prepare a single cell suspension in Cell Staining Buffer (BioLegend Cat. No. 420241). Note. If using in vitro stimulated cells, simply resuspend previously activated cultures in Cell Staining Buffer and proceed to Step 2. If using in vitro stimulated cells, simply ... rcs accor
immunofluorescent staining with flow cytometric analysis - BioLegend
WebStop cell lysis by adding 10ml Cell Staining Buffer to the tube. Centrifuge for 5 minutes at 350xg and discard supernatant. Repeat wash as in step 2. Count viable cells and … WebCell staining buffer is an antibody diluent and cell wash buffer optimized for use in immunofluorescent staining of viable or fixed single cell suspensions.Chemicals for use … WebElectron Microscopy Sciences (Hatfield, PA). Cell Staining Buffer (420241) was purchased from BioLegend (San Diego, CA). Nuclear GreenLCS1(17540)waspurchasedfromAATBioquest.MbCDwas purchased from Carbosynth (OD29274, 2,6-dimethyl-b-cyclodextrin). Hydroxypropyl- b-cyclodextrin (HPbCD) was … rcs-994