Galectin puncta assay
WebOct 21, 2024 · A galectin puncta assay involving double-immunostaining with anti-galactin-3 and anti-LAMP-2 Abs revealed that LMP, which is shown as colocalized puncta of galectin-3 and LAMP-2, was induced by LPZ + AZM combination treatment . Notably, treatment with either LPZ or AZM alone did not affect lysosomal galectin-3 puncta … WebApr 10, 2024 · In vitro infection assay. ... in PBS containing 3% BSA and 0.01% Triton X100 for 1 h. Next, coverslips were incubated with anti- LAMP1 and anti-Galectin-3 at 4 °C overnight. ... Confocal microscopy showed LC3B puncta (green) formation. LC3B puncta formation was quantified and graphically plotted. c Live cell imaging was performed using …
Galectin puncta assay
Did you know?
WebHere, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and …
WebOct 1, 2024 · Illustration of galectin puncta assay where cells may be stained with anti-galectin antibody to reveal either a cytosolic distribution of galectin-1, indicating no LMP or a punctate distribution of galectin-1, indicating LMP was induced. Galectin-1 has affinity to the glycosylated terminal of lysosome associated membrane protein 1 (LAMP 1 ... WebNov 15, 2024 · We used the galectin 3 puncta assay for early detection of LMP. Briefly, cytosolic galectin-3 binds to the glycocalyx in the luminal face of lysosomal membranes as they become leaky, which is detectable with anti-galectin, high‐affinity antibodies . We used L-Leucyl-L-Leucine methyl ester (LLOMe) treatment as LMP-positive control.
WebThe lysosomal galectin puncta assay is a sensitive method to detect LMP [17]. A strong galectin 1 signal was detected in siSHMT2 cells (Fig. 5A), suggesting LMP, resulting in the release of ... WebLC3 puncta formation was monitored by immunofluorescence staining. The interaction between indicated proteins was confirmed by co-immunoprecipitation (Co-IP) assay. Results: Either Galectin-3 or TRIM16 knockdown led to impaired ALP activity, reduced calcium deposition, down-regulation of pro-osteogenic markers as well as restrained …
WebNational Center for Biotechnology Information
WebExplore the latest full-text research PDFs, articles, conference papers, preprints and more on FLUORESCENT PROTEINS. Find methods information, sources, references or conduct a literature review on ... sandals cheap priceWebDec 29, 2024 · To assess the involvement of LMP in cell death induction kinetics, galectin-3 (Gal3) puncta assay was performed using A549 cells stably expressing Aequorea coerulescens green fluorescence protein ... sandals cheapest butler roomsWebMar 4, 2024 · Galectins are β-galactoside-binding animal lectins that contain at least one carbohydrate recognition domain (CRD) [].They can be categorized into three groups: (A) one-CRD-type, which can form homodimers, such as galectin-1; (B) two-CRD tandem repeat-type, which contains two different CRDs in tandem connected by a linker region, … sandals cheap vacationsWebAug 20, 2024 · The galectin puncta assay can be adapted for use with high-throughput imaging platforms in conjugation with other cell viability markers. 66. Finally, the ESCRT puncta assay used in a recent … sandals check availabilityWebDec 18, 2024 · The detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation. Expand. 231. PDF. sandals cheap shoesWebAug 1, 2024 · This view has, however, been challenged by the development of a galectin puncta assay, whose high sensitivity allows a reliable detection of a transiently leaky lysosome [6,7]. When the lysosomal membrane is damaged to the extent that permits lysosomal hydrolases with a molecular weight around 30 kDa to leak out, cytosolic … sandals cheap resortWeb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 sandals cheaper