Northernmax kit
WebThe Ambion ® NorthernMax ® Kit contains a complete set of RNase-free reagents for running formaldehyde-based northern analysis. This kit is ideal for the investigator who wants to use familiar reagents while taking advantage of NorthernMax ® Kit's high sensitivity. Sufficient reagents are provided to process 1000 cm2 membrane. …
Northernmax kit
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WebBiotin-HRP Northern blot was followed by manufacture’s manual (NorthernMax kit, Ambion) to demonstrate that all the RNAs are biotinylated and transcribed at the right size. 3. WebNorthernMax ™ –Gly Kit is an alternative to formaldehyde-based systems for northern analysis and uses a glyoxal/DMSO Loading Solution for sample denaturation. Sufficient …
WebThe NorthernMax™ Kit contains everything you need to conduct northerns. Positively charged nylon membranes must be purchased separately (see Accessory Products). An … WebNorthern blotting showed that transcripts for the zygote-specific molecule zys1A were up-regulated several fold during activation of HSV– gsp1 transformed gametes that had been mixed with imp1 mt+ gametes, whereas levels of zsp2 and Class VIII transcripts were unaffected by gamete activation.
Web23 de fev. de 2005 · Ambion's NorthernMax® Kit. Northern analysis is a standard laboratory procedure for quantitation and detection of mRNA following transfer and immobilization … WebThe NorthernMax® 10X Gel Running Buffer is a MOPS-based buffer (containing sodium acetate and EDTA) for use as a running buffer in denaturing formaldehyde agarose gel systems. MOPS is a zwitterionic buffer that serves as an electrolyte that favorably reduces heat generation while allowing timely separation to occur. Appearance: Clear solution.
Web1 de out. de 1999 · Total RNAs were extracted from each tissue using the RNeasy plant mini kit. Twenty micrograms of total RNA was used for blotting. The NorthernMax kit (Ambion, Austin, TX) was used for RNA gel blot hybridization, according to the manufacturer's instructions.
WebThe NorthernMax™ Kit contains a complete set of RNase-free reagents — including high quality agarose, gel loading solution, gel preparation and gel running buffers, transfer … irrational fear of being watchedWeb2 de jun. de 2008 · This kit is designed to produce short (<100 nt) labeled RNA transcripts for use in hybridization assays to detect small RNAs, including miRNA and siRNA. The kit supplies reagents for both transcription template preparation and RNA probe synthesis. Radiolabeled probes made with the kit are ideal for use with the mirVana miRNA … portable camping wood burnerWebThe NorthernMax™-Gly Kit can be used with either radiolabeled or nonisotopically labeled RNA or DNA probes. Each NorthernMax™-Gly Kit provides a complete system. … irrational fear of bugsWebI am using the Ambion NorthernMax-Gly kit with the biodyne B nylon membrane (positively charged), and the Chemiluminescent Nucleic Acid Detection Module for biotin detection, which is then ... portable camping washing machine south africaWebFunctional testing: NorthernMax® 10X Running Buffer is functionally tested using the Ambion® NorthernMax® Kit (Cat. no. AM1940). Limited product warranty Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and portable can opener walmartWebBrowse through our tips and tricks to set up your nucleic acid gel electrophoresis experiment for success. Find suggestions for agarose and acrylamide gels, as well as northern blotting. irrational fear of cockroachesWebNorthernMax™-Gly 试剂盒可以与放射性标记或非同位素标记的 RNA 或 DNA 探针一起使用。 每个 NorthernMax™-Gly 试剂盒都提供了一个完整的系统。 您必须单独购买带正电 … irrational fear of birds